The 第二道屏障的钥匙 is an Important Item in 异度神剑X. During 歼灭蒂法尼亚 it must be obtained along with the First and 第三道屏障的钥匙s to lower a forcefield. It can be found in 黑钢大陆's 格罗斯洞窟基地, on a raised area to the left of the entrance after leaving the tunnel.
Map showing the location (2) of the 第二道屏障的钥匙
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